Immobilized solid-phase DNA deamination for epigenetic sequencing of sparse DNA samples such as cell-free DNA.
Mutant TET enzymes that stall the oxidation reaction of 5-methylcytosine (5mC) at 5-hydroxymethylcytosine (5hmC), and AID/APOBEC enzymes with hyperactive deamination activity.
A split enzyme complex enabling precise gene editing
Method and probes for detecting and quantifying epigenetic regulator TET enzymatic activity. This method can be used for high throughput screening of TET inhibitors or activators.